IJE TRANSACTIONS B: Applications Vol. 15, No. 3 (October 2002) 227-234   

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G. D. Najafpour, A. Azizan and A. Harun

Department of Chemical Engineering, University of Sains, 14300 Nibong Tebal, S. P. S. 
Pulau Pinang, Malaysia, najafpour@hotmail.com - chghasem@eng.usm.my
amizon_azizan@hotmail.com - chazlina@eng.usm.my
( Received: October 29, 2001 – Accepted: April 03, 2002 )

Abstract    Biodesulfurization of coal has been carried out in batch culture of single strain and mixed culture of sulfur oxidizing bacteria. The pure cultures of Thiobacillus thiooxidans and Thiobacillus ferrooxidans utilized inorganic sulfur content of Malaysian coal. The chemolithotroph bacteria were able to grow on coal and metabolize the coal's sulfur content. The batch cultures of coal with 3-5 percent sulfur were used for growing microorganisms. A few species of bacteria and fungi had potential to utilize coal complex media, which were isolated from coal rice hulls and pharmaceutical wastewater. Asperigillus niger and Thiobacillus species isolated from coal, fungi from rice hulls and Saccharomyces species from wastewater, all microorganisms capable to grow on coal. The main concern was the sulfur oxidizing bacteria were screened out from all non-sulfur reducing bacteria. In the Batch cultures, the biooxidation coal's sulfur was affected by several parameters, such as pH, nutrients, agitation rate, growth stimulants and percentage coal. The trace amount of magnesium and cobaltions, 200 rpm agitation rate with initial pH of 2.6 for mixed culture of Thiobacillus thiooxidans and Thiobacillus ferrooxidans showed maximum growth rate and optimum batch operating conditions. The highest sulfur oxidation was obtained at the optimal Thiobacillus growth rate. More than 75 percent of sulfur was removed from coal. The biooxidation of coal, the kinetic model followed by Monod rate equation with specific growth rate, max = 4.8d-1 and rate constant. Ks =16.7 g/L.


Keywords    Biodesulfurization, Microbial Oxidation of Coal, Sulfur Removal, Thiobacillus Thiooxidans, Thiobacillus Ferrooxidans



1. Kargi, F. and Robinson, J. M., "Microbial Oxidation of Dibenzothiophene by the Hemophilic Organism Sulfblobus Acidocaldarius", Applied Biotechnology and Bioengineering, Vol. 27, (1984), 41-49.
2. Bozdemir, T. 0., Dumsoy, T., Erincin, E. and Yumm, Y., "Biodesulfurization of Turkish Lignite's", Fuel, Vol. 75, No. 13, (1996), 1596-1600.
3. Olson, G., Pott, B. M., Larssom, L., Hoist, 0. and Karlsson, H. T., "Microbial DesulfLirization of Coal Thiobacillus Ferrooxidans and Thermophilic Archaea", Fuel Processing Technology, Vol. 40, (1994), 277 -282.
4. Azizan, A., Najafpour, G. D., Hamn, A., "Microbial Desulfurization of Coal with Mixed Cultures of Thiobacillus Ferrooxidans and Thiobacillus Thiooxidans", Proceedings of the 2nd International Conference on Advances in Strategic Technologies-ICAST 2000, Putrajaya, Malaysia, ISBN: 983-9152-70-X, Vol. II, (15 - 17 August, 2000) 1457-1462.
5. Najafpour, G. D., Azizan, A. and Harun, A., "Biodesulfurization of Malaysian Coals using Mixed Microbial Cultures in Batch and Continuous Processes", International Conference on New Trends in Mineral Processing IV, VSB - T.U. of Ostrava, Ostrava, Czech Republic, ISBN: 80-7078-885-2, (June 2001), 289-296.
6. Azizan, A., Najafpour, G. D., Harun, A., "Biooxidation of Coal using Thiobacillus Ferrooxidans", University of Malaya, Ke-4, ISBN: 983-40344-0-7, (March 27-28, 2000), 98-107.
7. Szendroi, A., Gazso, L. G. and Hidvegi, E. J., "Biodesulfurization of Hungarian Lignite and Sub-bituminous Coal", Fuel Processing Technology, Vol. 40, (1994), 269-275.
8. Azizan A., Najafpour, G. D., Harun, A., "Biodesulfurization of Coal in Batch Culture", Regional Symposium on Chemical Engineering, RSCE 1999, B. P. Smila Beach Hotel, Songkhia, Thailand, Vol. I, A. (November 22 - 24, 1999), 491-496.
9. Mannivannan, T., Sandhya, S. and Pandey, R. A., "Microbial Desulfurization of Coal by Chemoautotrophic Thiobacillus Ferrooxidans - an Iron Mine Isolate", J. Environ, Sci Health, Vol. A29, No.10, (1994), 204-206.
10. Greenberg, A. E., Clesceri, L. S. and Eaton, A. D., "Standard Methods for the Examination of Water and Waste Water", American Public Health Association, American Water Works Association and Water Environment Federation, 8th Edition, Washington D. C., (1992).
11. Christian, G. D., "Analytical Chemistry", 143-145, John Wiley and Sons, New York, (1986).

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