Abstract




 
   

IJE TRANSACTIONS C: Aspects Vol. 28, No. 6 (June 2015) 841-847    Article in Press

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  BATCH KINETICS AND MODELING OF ALKALINE PROTEASE PRODUCTION BY ISOLATED BACILLUS SP. (RESEARCH NOTE)
 
G. Khayati and M. Zamani
 
( Received: February 27, 2015 – Accepted: May 02, 2015 )
 
 

Abstract    The aim of this study was the use of fish waste hydrolysate (FWH) as a substrate for alkaline protease production using isolated Bacillus sp. in a batch system. Then the fermentation kinetics of enzyme production was studied. The results show that with the addition of FWH to the fermentation medium with a final concentration of 4% (optimal concentration), alkaline protease value reached a maximum value (89 U/ml), which is 63% higher than that of the control medium. Also fermentation kinetics of alkaline protease by isolated Bacillus sp. was studied. Several kinetic models were evaluated; the combination of the Moser and Boulton kinetic model gave the best prediction. This nonlinear mathematical model performed satisfactory on biomass, substrate, and enzyme predictions.

 

Keywords    alkaline protease, Fish waste hydrolysate, modeling, Bacillus

 

چکیده    هدف از این مطالعه استفاده از هیدرولیز ضایعات ماهی (FWH) به عنوان یک سوبسترا برای تولید پروتئاز قلیایی و بررسی سینتیک تولید آنزیم با استفاده از جدایه باسیلوس در سیستم بسته بود. نتایج نشان داد که با با افزودن 4% FWH به محیط تخمیر حداکثر میزان تولید پروتئاز قلیایی به U/ml 89 رسید که آن 63٪ بالاتر از محیط کنترل بود. پس از بررسی چندین مدل سینتیکی ، مدل ترکیبی موزر و بولتون بعنوان بهترین مدل انتخاب شدند. نتایج نشان داد که یک سازگاری خوبی بین حل مدل و داده های تجربی برای غلظت سلول، سوبسترا و فعالیت آنزیم وجود دارد و این مدل ریاضی غیر خطی پیشگوئی زیست توده، غلظت سوبسترا و فعالیت آنزیم را بخوبی اقناع می کند. همچنین، ضریب همبستگی حاصله بالاتر از 98/0 بود ، به طوری که مدل سینتیکی می تواند برای شبیه سازی متغیرهای تخمیر مورد استفاده قرار گیرد.

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