Abstract




 
   

IJE TRANSACTIONS B: Applications Vol. 29, No. 2 (February 2016) 152-159    Article Under Final Proof

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  EXPERIMENTAL STUDY ON THE FACTORS AFFECTING HEXAVALENT CHROMIUM BIOREDUCTION BY BACILLUS CEREUS
 
M. Emadzadeh, M. Pazouki, E. Abdollahzadeh Sharghi and L.Taghavi
 
( Received: January 05, 2016 – Accepted: January 26, 2016 )
 
 

Abstract    Chromium through natural processes and human activities enters the air, soil and water. Chromium-resistant bacteria are capable of reducing toxic Cr(VI) to less toxic Cr(III). In this work, batch studies were conducted to evaluate the effect of environmental factors on the rate of Cr(VI) reduction from synthetic wastewater of metal plating industry by Bacillus cereus under aerobic conditions. The effect of different inoculum volumes (5, 10, 15 and 20 mL), pH (5, 7 and 9), temperatures (20, 30 and 40 °C) and initial concentrations of Cr(VI) (10, 50, 100 and 200 mg/L) for the best performance of chromium removal were investigated during 72 h of cultivation by Bacillus cereus. Complete reduction of Cr(VI) by Bacillus cereus was achieved after 48 h of incubation under optimized conditions of pH 9, inoculum volume of 5 mL, initial chromium concentration of 50 mg/L, and temperature of 40 °C. The results showed the highest rate of reduction at the lowest Cr(VI) concentration (1.04×10-2 h-1.10 mg/L) and the lowest at the highest Cr(VI) concentration (0.55×10-4 h-1.200 mg/L). Atomic absorption spectroscopy analyses under optimized conditions showed the concentration of Cr(III) in the culture supernatant was 49 mg/L after 48 h. The presence of almost all the reduced Cr(III) in the supernatant revealed Cr(VI)-reductase in Bacillus cereus is mainly associated with the soluble fraction of the enzyme. High Cr(VI) concentration resistance and high Cr(VI) reducing ability of Bacillus cereus make it a suitable candidate for bioremediation.

 

Keywords    Bioreduction of hexavalent chromium, Bacillus cereus, Metal plating industry, pH, Temperature, Inoculum volume, Cr(VI) concentration,

 

چکیده    کروم از طریق فرآیندهای طبیعی و فعالیت های انسان وارد هوا، خاک و آب میشود. باکتری های مقاوم به کروم قادر به کاهش کروم (VI) سمی به کروم (III) با سمیت کمتر می باشند. در این کار، مطالعات ناپیوسته به منظور بررسی اثر عوامل محیطی بر میزان نرخ کاهش کروم (VI) از پساب سنتزی صنعت آبکاری فلز توسط باسیلوس سرئوس در شرایط هوازی انجام شد. اثر حجم های مختلف مایه تلقیح (5، 10، 15 و 20 میلی لیتر)، pH (5، 7 و 9)، درجه حرارت (20، 30 و 40 درجه سانتی گراد) و غلظت های اولیه کروم (VI) (10، 50 ، 100 و 200 میلی گرم بر لیتر) برای بهترین عملکرد حذف کروم در طول 72 ساعت مورد بررسی قرار گرفت. کاهش کامل کروم (VI) توسط باسیلوس سرئوس بعد از 48 ساعت انکوباسیون تحت شرایط بهینه 9 pH، حجم مایه تلقیح 5 میلی گرم، غلظت اولیه کروم 50 میلی گرم بر لیتر و درجه حرارت 40 درجه سانتی گراد به دست آمد. نتایج نشان داد که بیشترین نرخ کاهش در کمترین غلظت کروم (VI) (mg/L 10. h-1 2-10× 04/1) و کمترین نرخ کاهش در بیشترین غلظت کروم (VI) (mg/L 200. h-1 4-10× 55/0) بود. آنالیز جذب اتمی در شرایط بهینه نشان داد که غلظت کروم (III) در مایع رویی کشت بعد از 48 ساعت 49 میلی گرم بر لیتر بود. حضور تقریبا تمام کروم (III) کاهش یافته در مایع رویی نشان داد که کروم (VI)-ردوکتاز در باسیلوس سرئوس به طور عمده با بخش محلول آنزیم همراه است. مقاومت در برابر غلظت بالای کروم (VI) و توانایی کاهش بالای کروم (VI) باسیلوس سرئوس، آن را یک گزینه مناسب برای تصفیه زیستی می سازد.

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