IJE TRANSACTIONS A: Basics Vol. 29, No. 4 (April 2016) 436-443   

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Z. Kouchak Yazdi and I. Alemzadeh
( Received: March 06, 2016 – Accepted in Revised Form: April 14, 2016 )

Abstract    Conjugated Linoleic Acid (CLA), Glycerol (G) and sunflower oil blends with varying concentration were subjected to enzymatic esterification using a 1, 3- specific immobilized lipase. CLA was used as acyl due to its purported health benefits. The transesterified lipids were evaluated for free fatty acids (FFA) and composition of fatty acids by gas chromatography. Lipozyme RM IM is preferred for enzymatic esterification because of more CLA incorporation and less free fatty acids at the end of reaction than Lipozyme TL IM. Response surface methodology (RSM) was used to determine the effects of three variables Glycerol concentration, reaction temperature, and amount of enzyme on the lipase catalyzed incorporation of CLA into Sunflower oil. The optimum condition for maximum CLA incorporation (96.7% Incorporation Yield), maximum reaction rate (0.0271 M/h) and minimum free fatty acids production (Acid No.2.73) was obtained, at 75 ̊C by using 3 % enzyme and 0.27 M glycerol.


Keywords    Conjugated linoleic acid, Response surface methodology, Sunflower oil, Immobilized lipase


چکیده    [if gte mso 9]> مخلوط اسيد لينولئيک مزدوج CLA، گلیسرول و روغن آفتابگردان با ترکیب درصدهای مختلف جهت انجام واکنش استریفیکاسیون آنزیمی به وسیله لیپاز تثبیت شده با انتخاب پذیری جایگاه 1و3 استفاده شد. اسيد لينولئيک مزدوج به سبب خواص بيولوژيکي بسيار مفيد استفاده شد. ترکیب اسیدهای چرب نمونه­های نهایی پس از فرآیند استری شدن توسط کروماتوگرافی گازی به دست آمد. با توجه به نتایج آنزیم لیپوزیم RM IM و جذب بیشتر CLA به بدنه تریگلیسرید و کاهش قابل توجه اسیدیته مخلوط واکنش در طول انجام واکنش جهت انجام این واکنش ارجحیت دارد. روش پاسخ سطحی جهت بررسی اثرات متغییرهای غلظت گلیسرول، دمای واکنش و مقدار آنزیم به کار گرفته شد. در بهينه سازي فرآيند براي توليد روغنی با حداکثر CLA جذب شده، حد اکثر سرعت واکنش، حداقل اسيدهاي چرب آزاد و به حداقل رساندن افت روغن، شرایط بهینه در دمای75 درجه سانتی­گراد و با استفاده از 3 درصد آنزیم و 0.27(M) گلیسرول با 96.7% بازده جذب CLA به دست آمد.


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