IJE TRANSACTIONS B: Applications Vol. 30, No. 8 (August 2017) 1110-1117   

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N. Noshadi, M. Mohammadi, G. D. Najafpour and F. Pouryafar
( Received: March 19, 2017 – Accepted in Revised Form: July 07, 2017 )



Keywords    α-Amylase; Biomass hydrolysate; Bacillus amyloliquefaciens; Submerged culture; Enzyme activity


چکیده    در این پژوهش، آنزیم آلفا-آمیلاز مقاوم در برابر حرارت با استفاده از ضایعات لیگنوسلولزی توسط باکتری باسیلوس آمیلولیکوفسینس تولید شد. برای این منظور، از محلول حاصل از هیدرولیز سبوس گندم، سبوس برنج و تفاله نیشکر به عنوان سوبسترا برای تولید آنزیم استفاده گردید. بیشترین میزان تولید آنزیم در محیط کشتی بود که حاوی عصاره هیدرولیز سبوس گندم بود. برای افزایش میزان تولید آلفا-آمیلاز، محیط کشت از نظر میزان مکمل‌های کربنی و نیتروژنی بهینه‌سازی شد. فعالیت آنزیمی در محیط کشت بهینه (94/208 واحد بر میلی‌لیتر) بسیار بالاتر از محیطی بود که بهینه‌سازی در آن انجام نشده بود (22/76 واحد بر میلی‌لیتر). فعالیت و پایداری آنزیم سنتز شده در محیطهای مختلف با دما و pH متفاوت بررسی شد. شرایط بهینه برای داشتن بیشترین فعالیت آنزیمی (pH برابر با 7 و دمای 70 درجه سانتی‌گراد) و بالاترین پایداری (pH برابر با 7 و دمای 50 درجه سانتی‌گراد) تعیین شد. تاثیر حضور یونهای فلزی مختلف روی فعالیت آنزیمی بررسی گردید. فعالیت آنزیمی در حضور یونهای منیزیم، منگنز، روی، سدیم، مس و کلسیم بهبود یافت در حالی که یون آهن فعالیت آنزیمی را کاهش داد.


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