IJE TRANSACTIONS B: Applications - Special Issue - Sustainable Technologies for Water and Environment; Guest Editor Prof. Dr. Ahmad Fauzi Ismail and Associate Guest Editor Dr. Lau Woei Jye, Universiti Teknologi Malaysia (UTM), Malaysia
Vol. 31, No. 8 (August 2018) 1308-1317    Article in Press

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N. S. Md Norodin, L. Md Salleh, N. M. Mustafa, N. Yusof, F. Kamarulzaman, M. A. Mohamed Zahari and N. A. Bakeri
( Received: December 16, 2017 – Accepted: February 08, 2018 )

Abstract    This study analyzed the inhibition activity of Swietenia mahagoni seeds extract on α-glucosidase and α-amylase enzymes inhibition assays. Swietenia mahagoni seeds were extracted by using Supercritical Carbon Dioxide (SC-CO2) extraction at pressures of 20- 30 MPa and temperatures of 40- 60°C. The oil yields obtained were analyzed with α- glucosidase and α- amylase enzymes inhibition assays. All data obtained were expressed as mean ± standard deviation for triplicate experiments. One way analysis was used for statistical significance by using Statistica software version 7.0 (StartSoft, EUA) and IC50 (extract concentration causing 50% enzyme inhibitory) was determined by using GraphPad Prism 6.0 software. Swietenia mahagoni seeds extract have a strong inhibition of α-glucosidase enzyme activity (98.4% ± 0.2) but a moderate inhibition of α- amylase enzyme activity (34.9% ± 1.2). These findings implied that Swietenia mahagoni seeds extract could be an effective natural antidiabetic agent.


Keywords    Swietenia mahagoni seed, α-glucosidase enzyme, α-amylase enzyme, Supercritical Carbon Dioxide (SC-CO2) extraction



مطالعه حاضر به بررسی فعالیت مهارکننده استخراج عصاره گیاه ماهون در آزمایش مهار آنزیم α گلوکز و α آمیلاز میپردازد. عصاره گیاه ماهون با استفاده از دی اکسید کربن فوق بحرانی با فشار 30ـ20 مگاپاسکال و دماهای 60ـ40 درجه سانتیگراد استخراج میشوند. بازدهی روغن حاصل از آنالیز با آنزیم α گلوکز و α آمیلاز آنالیز می شود. تمامی داده ها در قالب میانگین انحراف معیار برای آزمایشهای سه گانه تفسیر شده اند. از نرم افزار Statistica نسخه 7 برای معنی دارشدن آماری آنالیز یکطرفه استفاده شد و غظلت عصاره 50 درصد آنزیم مهارکننده با استفاده از نرم افزار GraphPad Prism 6.0 تعیین شد. قدرت مهارکنندگی عصاره گیاه ماهون در فعالیت آنزیم α گلوکز (98.4%0.2) شدید و در فعالیت آنزیم α آمیلاز متوسط (34.9%1.2) است. این یافته ها نشان میدهد که عصاره گیاه ماهون میتواند ماده ضد دیابت طبیعی موثر باشد.

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